Detail Cantuman

Honey was a natural product derived from insects and flower nectar. Honey has natural antibiotics that are beneficial to health. Honey contains flavonoid and phenolic compounds that act as antioxidants, an antimicrobial agent. The purpose of this study was to determine the Minimum Inhibitory Concentration of 4 types of honey against Escherichia coli, Salmonella typhi, and Staphylococcus aureus bacteria and to determine the antioxidant activity. The experimental design was Completely Randomized Design (CRD) with the first factor being Apis mellifera, Apis dorsata,Apis mellifera (bunga Randu) and Trigona sp honey. The second factor was Escherichia coli, Salmonella typhi, and Staphylococcus aureus as tested bacteria. The MIC determination method according to Kirby Bauer which was modified using a UV-Vis Spectrophotometer. The MIC results of Apis mellifera honey and Apis mellifera (Randu flower) against Escherichia coli, Salmonella thypi, and Staphylococcus aureus bacteria were 50% (v/v), in Apis dorsata honey against Escherichia coli, Salmonella thypi bacteria were 6.25% (v/v), while the bacteria Staphylococcus aureus 12.5%(v/v) and the type of honey clanceng against Escherichia coli and Staphylococcus aureus bacteria was 12.5%(v/v) Salmonella typhi bacteria 6.25% (v/v). The highest antioxidant activity was in the type of Trigona sp honey with a value of 49%.